Test Directory

7317 Vitamin D, 25-hydroxy Total

Specimen Type
Specimen Stability
Reference Range
21 Days, Frozen
21 Days
Ambient-21 Days
See Report

Specimen Requirements

Specimen Type: Serum
Container: 1 Serum Separator Tube (SST)
Temperature: Refrigerated
Preferred Vol: 1.00 mL
Min Vol: 0.50 mL


Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)


Sunday - Saturday

Turn-Around Time



Clinical Utility

Measurement of the 25-hydroxyvitamin D [25(OH)D] blood level is the accepted means for determining vitamin D status (N Engl J Med. 2007;357:266-281). Such measurements provide a good index of circulating vitamin D activity in patients not suffering from from renal disease. Lower than normal 25(OH)D levels can result from insufficient sun exposure, a dietary deficiency, poor absorption of the vitamin, or impaired metabolism in the liver. A 25(OH)D D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead hypercalcemia. This assay assay employs liquid chromatography, tandem mass spectrometry to independently measure and report the two common forms of 25(OH)D: 25(OH)D3 and 25(OH)D2.

CPT Codes


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